CG200
 |  |  |
 |
Image
Useful Links & Tools
- MSDS
- Certificate of Analysis
- Certificate of Origin
- CG200 - Bulletin (131 KB)
- Bulk Quote
- Similar Products
CG200
Sigma
cGMP Enzyme Immunoassay Kit, Direct
sufficient for 96 assays
Description
| Application | Non-radioactive, competitive immunoassay for the quantitation of total cGMP in tissue and cell cultures. Buffer contains 0.1N HCl that aids in the lysis of cells, inhibits endogenous phosphodiesterases and stabilizes the cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well. |
Properties
| usage | sufficient for 96 assays |
| shipped in | wet ice |
| storage temp. | −20°C |
Safety
| Hazard Codes | F,C |
| Risk Statements | 11-14-20/21/22-35 |
| Safety Statements | 16-26-36/37/39-45 |
| RIDADR | UN 1715 9/PG 3 |
Components
| Standard component | 0.1 M Hydrochloride Acid 1×30 mL |
| Acetic Anhydride 1×1 mL | |
| cGMP-Alkaline Phosphatase Conjugate 1×5 mL | |
| cGMP EIA Antibody Rabbit Anti-cGMP 1×5 mL | |
| Cyclic GMP Standard 1×0.5 mL | |
| Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 each | |
| Neutralizing Reagent 1×6 mL | |
| p-Nitrophenyl Phosphate Substrate Solution 1×20 mL | |
| Plate Sealer 1 each | |
| Stop Solution 1×5 mL | |
| Triethylamine 1×2 mL | |
| Wash Buffer Concentrate 1×30 mL |
References
| reference | Chard, T. An Introduction to Radioimmunoassay and Related Techniques 4th ed., Amsterdam , (1990) |
| Tijssen, P. Practice and Theory of Enzyme Immunoassays Amsterdam , (1985) |
